Korea Basic Science Institute

COMMODITY DESCRIPTION

Commodity

HR/AM(High Resolution Accurate Mass) Integrated Omics Mass Analyzer

HSK No.

9027.80.1000

Page  page  of  pages

Date

2014.02.12

Description

Unit

Q'ty

A. Features

1. The instrument is a high resolution and accurate mass fourier transform mass spectrometer system with ultra high resolution performance for Genomics, Proteomics, and Metabolomics analysis.

2. The instrument has a high resolution scan mode, more than R=100,000 , for the accurate mass measurement and the analysis of the multiple charged ions.

3. The instrument should combines the high throughput MS/MS system with ultra high resolution fourier transform mass analyzer into a single instrument for unprecedented analytical power and versatility.

4. The multiple fragmentation(MSn) and high energy collisional dissociation(HCD) must be performed simultaneously for various omics analysis.

5. All of the ion optics are controlled through the data system for automatic tuning and optimization.

6. Integrated switching Valve and Syringe Pump are controled by data system.

7. The system is fully controlled and all data processing carried out using a single personal computer

B. System Configuration

1. Hybrid advanced mass analyzer with FT MS system

1) Advanced mass analyzer or triple quad MS

2) High resolution FT-MS

2. Split-free nano LC

3. Data system

4. ETD or multiple charged Intact Protein mode

5. Accessories

C. Specification

1. Hybrid advanced mass analyzer with FT MS system

** Performance Specifications **

A. Mass Resolution : > 280,000 at m/z 200

B. Mass Accuracy : < 3 ppm RMS with external calibration

< 1 ppm RMS using internal calibration

C. Mass Range : up to m/z 6,000

D. Isolation width : < 0.3 Da

E. Dynamic Range : > 5,000 within a single scan guaranteeing

specified mass accuracy

F. MS Scan Power : >12Hz high resolution full scan MS/MS

G. MS/MS Sensitivity : > 100:1 S/N ratio with 100fg Rserpine or Ubiquitin

1) Advanced mass analyzer or triple quad MS

(1) Transfer Ion Optics

a. Advanced transfer lense

b. High stability and ion transmission efficiency

(2) 2D Linear Ion Trap or Triple Quad

a. Analyzer dimensions optimized for ultimate performance

b. Regulated helium flow for stable performance

c. Automatic system calibration and optimization

(3) Vacuum System

a. Differentially-pumped vacuum system

b. Split-flow turbomolecular pump controls vacuum in three regions

c. Dual rotary vacuum pump configuration

d. High-vacuum machined aluminum analyzer chamber

(4) Detection System

a. Dual +/-15KV conversion dynode detector.

b. Two off-axis continuous dynode electron multipliers with extended

dynamic range

c. The instrument must be capable of switching polarities within 100ms

(5) Integrated Divert Valve

a. Fully-automated data system control enables user to switch the

solvent front, gradient end point and any other portion of

the HPLC run to waste

(6) Integrated Syringe Pump

a. Syringe Pump allows automated infusion under data system control

(7) High-Performance API Nanospray Source

a. The API source must have flat tuning and must have ion-optics that enable automated tuning.

b. Must include an independently heated ion capillary tube, heated by an external heater capable of temperatures up to 400℃.

c. The ion transfer tube must be removable to provide vent-free maintenance.

d. The nanospray ion sources include CCD camera and can be compatible with liquid flow rates of 50 nL/min to 2 μL/min.

e. The API source must incorporate a safety interlock switch to shut off voltages when probe is removed from source housing

f. One flexible platform nanospray ion source supports X,Y,Z axis adjustment and liquid junction for various applications.

(8) Scan Function

a. MSn for multi-stage MS experiments to probe the structure of ions

b. High resolution isolation allows for the separation of an isobaric interfering species down to 0.3 Da or for isolation of a thermally labile compound.

c. Automatic Gain Control and Predictive AGC ensures that the ion trap is always filled with the optimum number of ions for any scan type

d. Dynamic Exclusion allows acquisition of MS/MS and MSn spectra from lower intensity ion species

e. Normalized Collision Energy compensates for the mass-dependent energy deposition characteristics of ion trap mass spectrometers in MS/MS experiments, providing reproducible data from instrument to instrument.

f. Data Dependent features trigger acquisition of MSn spectra only when a compound of interest is detected.

g. Data-independent analysis(DIA) and parallel reaction monitoring (PRM) for high throughput reproducible quantitation.

2) High resolution FT-MS

(1) The mass analyzer is the most advanced FT-Orbitrap or FT-ICR mass analyzer technique with ultra high resolution and high mass accuracy.

(2) The electrostatic ion trap or ion cyclotron resonance trap with gas free multipole ion transfer optics.

(3) The electrostatic ion trap or ion cyclotron resonance trap with gas free multipole ion transfer optics.

(4) Improved gas filled C-Trap and ion transfer optics to the Orbitrap analyzer.

(5) Straight multipole collision cell with axial field for Higher Energy Collisional Dissociation (HCD).

(6) Improved low noise detect amplifier and 14 bit signal digitization.

(7) Improved ultra fast real-time data acquisition and instrument control system

(8) Differentially pumped vacuum system with ;

a. One split-flow turbo-molecular pump (at linear ion trap), three 60 L and one 210 L turbo molecular pumps.

b.	Pressure in the Orbitrap, under operating conditions, better than 4 x 10-10 mtorr.

c.	Rotary vane pump as fore vacuum pump.

d.	Penning Ion gauge and cold cathode gauge, both with electronic monitoring.

2. Split-free nano LC

1) High performance Nano Pump (Gradient pumps and loading pump)

(1) Flow rate ranges : Nano Pump: 20 nL/min–50 μL/min using

pre-calibrated flow meter cartridge

(2) Pressure range : 2–80 MPa (11,600 psi, nano pump)

(3) Number of solvents channels : Two (nano pump)

(4) Retention time RSD in gradient mode at 300 nL/min :

< 0.10 min SD or < 0.2% RSD (typically < 0.10% RSD)

(5) Flow Calibration : Semi-automate

(6) Proportioning Accuracy : <1% of full scale

(7) Proportioning Precision : Typically <0.2% SD

(8) Retention Time RSD in Gradient Mode at 300 nL/min:

<0.2% RSD or <0.1 min SD

(9) Gradient delay volume : < 25 nL (pump)

(10) Flow rate precision : < 0.1% RSD at 300 nL/min and 40 MPa

(11) Eluent bottles : 2 × 100 mL

2) Thermostatted Column Compartment

(1) Recommended eluent pre-heater volume : 2 μL (0.005": i.d.)

(2) Temperature range : 5 °C to 110 °C (max. 18 °C below ambient)

(3) Temperature accuracy : ± 0.5 °C

(4) Temperature stability/precision : ± 0.1 °C

(5) Heat-Up/cool-down time : Typically: 15 min from 20 °C to 50 °C

3) Nano Autosampler

(1) Sample capacity :

216 × 0.3 mL vials,

120 × 1.1 mL conical vials,

216 × 1.2 mL vials,

120 × 1.8 mL/2.0 mL vials,

66 × 4 mL vials,

30 × 10 mL vials,

3 x 24 deep well plates, 96 and/or 384 normal or deep well plates,

(2) Injection methods : Full-loop and partial-loop injections, low dispersion mode, microliter pickup, user-defined programs

(3) Injection volume range : 0.001–5 μL (recommended: 20 nL–5 μL)

Up to 125 μL (optional)

(4) Injection Technique : Needle in needle, with programmable needle wash Pulled loop mechanism

(5) Maximum pressure : 1000 bar (14,500 psi)

(6) Minimum sample required : 1 μL out of 1 μL (microliter pickup)

(7) Injection volume precision : 0.4% RSD at 1 μL in full-loop and

< 1% RSD at 200 nL partial-loop mode, caffeine in water

(8) Linearity : Corr. coeff. >0.9995 at 100–500 nL, partial-loop injection, caffeine in water

(9) Needle wash carry over : Active external needle wash,

<0.02% for caffeine with external wash

(10) Injection cycle time : <30 s for 1 μL full-loop injection

(11) Sample thermostatting : 4–45 °C or 22 °C below ambient (thermostatted sampler versions)

(12) Dimensions(h × w × d) : 36 × 42 × 51 cm (14.2 × 16.5 × 20 in.)

4) Nano LC start-up kit

(1)  column, 5 mm, C18, 15cm x 75mm , 1ea

(2)  trap column holder, 1ea

(3)  trap column 5mm C18, 2.5cm x 100um, 1ea

(4)  nano spray tip 360/20mm ID/OD, 10mm tip , 1pk

(5) peptide standard, 1ea.

5) Accessories for nano-LC

(1) Solvent rack with solvent reservoir, 1set

(2) Direct Injection nano LC kit, 1 set

(3) Line Cord, 3COND, 4ea

(4) Solvent waste/seal rinse bottle, 250mL, 2ea

(5) Flow meter calibration assembly for channel 2, 1ea

(6) Flow meter calibration assembly for channel 1, 1ea

(7) Transfer capillary, 360um OD, 25um ID, 2m

(8) Transfer capillary, 360um OD, 50um ID, 2m

(9) Transfer capillary, 360um OD, 100um ID, 2m

3. Data system

1) PC data system hardware with Intel Xeon 2.40 GHz processor :

(1) 256 MB PCIe x16 nVidia,Quadro NVS 295

(2) 8 GB DDR3 SDRAM

(3) 1 TB SATA 3.0Gb/s with NCQ and 16 MB DataBurst Cache

(4) 16 x DVD writer (+/-RW)

(5) 10/100/1000 Gigabit Ethernet controller PCI Express

(6) Windows 7 operating system

(7) Microsoft Office 2010, Norton 2003 anti-virus software, or equivalent

(8) 24 inch LCD monitor

2) High performance data processing hardware with ;

(1) Intel i7 Haswell 4770K (3.5 GHz/8MB/LGA1150)

(2) CORSAIR Dominator Platinum DDR3 (8G x 2) PC3-28000/1600/CL9

(3) ASUS MAXIMUS 6 FORMULA (Z87)

(4) Inno3D GTX770 iChiLL D5 2GB HerculeZ X3 Ultra

(5) SSD 840 Pro 512GB, MZ-7PD512B/KR

(6) HDD 2TB (SATA3/7200/64M) x 2ea

(7) 16 x DVD writer (+/-RW)

(8) Windows 7 operating system

(9) MS Wireless Comfort Desktop 5000 set

(10) CORSAIR Professional series 860 80PLUS platinum 파워

(11) 3R L700 이클립스 블랙 케이스

(12) 27 inch LCD monitor

3) Data system software for instrument control and data evaluation

(1) Fully integrated user interface for instrument tuning, set-up and data acquisition

(2) Dynamic exclusion capability to temporarily exclude a precursor ion while allowing acquisition of MS/MS spectra; increases the selectivity of co-eluting compounds, and enhances the signal of a selected compound

(3) Trigger functionality for data dependent experiments to execute the trigger close to the apex of a chromatographic peak resulting in fast and high quality MS/MS spectrum quality

(4) Isotopic Data Dependence scan capability to automatically perform MS/MS when a user-definedisotopicpatternisdetected

(5) Isotopic Tag data dependency scan capability to automatically perform MS/MS when a user-defined mass difference is recognized between two signals.

(6) Neutral loss data dependency scan capability to automatically perform MS/MS when a user-defined m/z loss is recognized between two signals in full MS and all ion fragmentation mode.

(7) Normalized collision energy capability for HCD, to automatically compensate for the mass dependent energy deposition and provide instrument-to-instrument reproducibility

(8) Automatic gain control to automatically control ion injection time for optimized ion current across the entire chromatographic peak

4. ETD or multiple charged Intact Protein mode

1) Identifies both the type and site of PTMs

2) Alternating ETD and CID data scans provide greater protein sequence coverage

3) Compatible with proton transfer reaction chemistry

4) Multi-Algorism Program for Global Proteomics: Sequest, Mascot and Z-core

5) Glycan-/Glycopeptide Identification

6) Top-Down Proteomics

D. Standard Acc's

1) Ultra high resolution mode

2) Protein ID/Quan software

3) De novo sequencing software

4) Installation & Maintenance kit

5) 75/100um tubing, 30m/roll

6) C18, 5u/3u, 100Å resin

7) NanoLC trap column, 2.5cm x 100um, 3/pk

8) Blank nano spray tip 10.5cm x 20/pk

9) Differential expression software

10) Metabolite ID and spectral interpretation software

11) 175 LN2 and He container with regulator

12) LC/MS table

13) sample vial 0.25ml C/T PP 12x32, 1,000ea/pk

14) 11mm aluminum cap PTFE/SIL, 1,000ea/pk

15) sample vial rack

16) heating block(24~48 hole, 30~95℃)

E. Remarks

1. This installation and operation should be carried out by the bidder/supplier's responsibility at end user's site.

2. Two years hardware warranty and three years software warranty should be provided by supplier after the date of installations.

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